EC Number |
Reference |
---|
5.3.3.2 | - |
660922 |
5.3.3.2 | comparison of orthorhombic, monoclinic and trigonal crystal forms, up to 2.2 A resolution. Crystallization of free enzyme and in complex wih transition-state analogue N,N-dimethyl-2-amino-1-ethyl diphosphate |
690288 |
5.3.3.2 | crystal structure of free and metal-bound C67A mutant enzyme |
650979 |
5.3.3.2 | crystal structure of the isomerase-bromohydrin complex |
651594 |
5.3.3.2 | crystallized at 20°C using the hanging-drop vapor diffusion method with a reservoir solution containing 0.1 M Tris-HCl (pH 8.0), 0.2 M sodium citrate, and 30% (vol/vol) polyethylene glycol 400 (PEG 400) |
719740 |
5.3.3.2 | crystallographic investigation of phosphoantigen binding |
661948 |
5.3.3.2 | crystals soaked with transition state analogue (N,N-dimethylamino)-1-ethyl diphosphate |
680346 |
5.3.3.2 | enzyme shows a flexible N-terminal alpha-helix covering the active pocket and blocking the entrance. Substrate binding induces conformational change in the active site. A water molecule is the direct proton donor for the substrate |
681412 |
5.3.3.2 | hanging drop vapor diffusion method, crystal structure of the C67A mutant of isopentenyl diphosphate isomerase complexed with the mechanism-based irreversible inhibitor 3,4-epoxy-3-methyl-1-butyl diphosphate |
653840 |
5.3.3.2 | hanging drop vapor diffusion method, crystal structures of complexes with transition state analogue N,N-dimethyl-2-amino-1-ethyl diphosphate and the covalently attached irreversible inhibitors 3,4-epoxy-3-methyl-1-butyl diphosphate at 1.96 A resolution |
652367 |