EC Number |
Cofactor |
Reference |
---|
3.4.21.91 | more |
characterization of structural determinants necessary for activation of the enzyme by the NS2B cofactor. Alanine substitutions at residues Trp62, Leu75, and Ile79 in the NS2B cofactor result in marked effects on autoprocessing at the NS2B/NS3 site. The activity of the mutant NS3 protease with the synthetic peptide substrate Gly-Arg-Arg-4-methylcoumarin 7-amide is mainly affected by significantly reduced kcat values |
666187 |
3.4.21.91 | more |
chimeric proteases with the NS2B cofactor from Langat virus and Dengue virus type 3 replacing the Alkhurma virus sequence activate NS3pro |
681170 |
3.4.21.91 | NS2B cofactor |
- |
752665, 754838, 755540, 755564 |
3.4.21.91 | NS2B cofactor |
essential |
753571, 755305 |
3.4.21.91 | NS2B cofactor |
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease |
752513 |
3.4.21.91 | NS2B cofactor |
protein essential for activity |
753569 |
3.4.21.91 | NS2B cofactor |
protein required for proteolytic activity |
752790 |
3.4.21.91 | NS2B cofactor |
The dengue virus NS2B-NS3 protease (NS2B-NS3p), an important antiviral target for drug development, has been reported to adopt an open or closed conformation in crystal structures with different NS2B C-terminus (NS2Bc) positioning. NS2Bc departs from NS3p in the open conformation, but it forms tight interactions with NS3p in the closed conformation |
752653 |
3.4.21.91 | NS2B cofactor |
the NS2B cofactor is covalently linked to the NS3 protease domain via a Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly (G4SG4) linker |
752666 |
3.4.21.91 | NS2B cofactor |
the unique cofactor region of zika virus NS2B-NS3 protease facilitates cleavage of key host proteins. The NS2B cofactor region from Zika virus protease is essential for recognition of host cell substrates. Replacing the NS2B region in another flavivirus protease enabled recognition of novel Zika-specific substrates by hybrid proteases, suggesting that the cofactor is the principal determinant in ZVP substrate selection |
752362 |