   2.6.1.B16 | pyridoxal 5'-phosphate |
dependent on. Many PLP-dependent enzymes, including transaminases, require the formation of a so-called internal aldimine (E-PLP), before the main reaction. In this process, the PLP coenzyme becomes covalently bound to an active-site lysine residue as a protonated Schiff base, which constitutes the active form of the enzyme. The transamination reaction consists of two half-transamination equilibria. An amino donor is converted to the corresponding ketone or aldehyde, resulting in the conversion of E-PLP to pyridoxamine-5'-phosphate (PMP). The E-PLP is then regenerated by the reaction of another ketone or aldehyde, which is then converted into an amine |
758577 |
