EC Number |
Cofactor |
Reference |
---|
2.5.1.44 | NAD+ |
- |
288698, 288700, 288701, 288702, 704662 |
2.5.1.44 | NAD+ |
absolute requirement |
288698 |
2.5.1.44 | NAD+ |
cannot be replaced by NADP+, NADPH, NADH |
288697 |
2.5.1.44 | NAD+ |
NAD+ seems to function as hydride acceptor in the first part of the reaction and subsequently as hydride donor in the second part |
288699 |
2.5.1.44 | NAD+ |
required in catalytic amounts with a Km-value of 0.0025 mM |
288697 |
2.5.1.44 | NAD+ |
unique usage of NAD(H) as a prosthetic group. the cofactor is coordinated through hydrogen bonding via residues Ser21, Ile22, Ser230 (phosphate), Asp45, Val66 (adenosine), Ser92,Thr114, Ala161, Asn162, and Pro163 (nicotineamide riboside). The phosphate-binding motif (18GFGSIG23) is located in the loop connecting beta-strand 2 and alpha-helix A of the Rossmann fold. The adenosine part of NAD+ is bound via loop regions located between beta-strand 4, 5, 6 and alpha-helix C, D, E. Nicotineamide-riboside-binding residues are found in loop regions between beta-strand 7 and 8 and alpha-helix F and O |
739672 |
2.5.1.44 | NADH |
unique usage of NAD(H) as a prosthetic group. the cofactor is coordinated through hydrogen bonding via residues Ser21, Ile22, Ser230 (phosphate), Asp45, Val66 (adenosine), Ser92,Thr114, Ala161, Asn162, and Pro163 (nicotineamide riboside). The phosphate-binding motif (18GFGSIG23) is located in the loop connecting beta-strand 2 and alpha-helix A of the Rossmann fold. The adenosine part of NAD+ is bound via loop regions located between beta-strand 4, 5, 6 and alpha-helix C, D, E. Nicotineamide-riboside-binding residues are found in loop regions between beta-strand 7 and 8 and alpha-helix F and O |
739672 |
2.5.1.44 | NADP+ |
68% of the activity with NAD+ |
288702 |