EC Number |
Cofactor |
Reference |
---|
1.8.1.B1 | FAD |
- |
724324, 724422, 724508, 725454, 725773, 741532, 741620, 763906 |
1.8.1.B1 | FAD |
each catalytically active unit consists of three redox centers: FAD and an N-terminal Cys57/Cys62 redox-active disulfide from one monomer and a Cys489/Cys490 C-terminal redox-active disulfide from the second monomer |
685204 |
1.8.1.B1 | FAD |
flavoprotein |
764019 |
1.8.1.B1 | FAD |
the FAD content of the recombinant enzyme is approximately 25% |
724926 |
1.8.1.B1 | FAD |
the flavin ring of FAD cofactor binds in a hydrophobic cleft formed by Cys154, Val157, Gly158, Cys159, Pro572, Phe474, and Pro443 |
742689 |
1.8.1.B1 | NADP+ |
- |
724508 |
1.8.1.B1 | NADPH |
- |
724324, 724422, 724926, 725454, 725773, 726093, 726293, 726313, 741620, 742460, 743070, 743291, 743395, 743398 |
1.8.1.B1 | NADPH |
nicotinamide moiety binds up in the hydrophobic cleft formed by Val348, Ile266, Pro264,Val292, Met315, Val316, and Pro349, etc. and the diphosphate forms H-bond with Ile391, Ser318, and Arg317. The ribose sugar formes H-bonds with Arg262, Arg322, and Tyr296 |
742689 |