EC Number |
Cofactor |
Reference |
---|
1.5.1.5 | more |
enzyme MTHFD2L uses both NAD+ and NADP+ for methylenetetrahydrofolate dehydrogenase activity but requires both phosphate and Mg2+ when using NAD+ |
742856 |
1.5.1.5 | more |
the enzyme MTHFD2L uses both NAD+ and NADP+ for methylenetetrahydrofolate dehydrogenase activity but requires both phosphate and Mg2+ when using NAD+ |
742856 |
1.5.1.5 | more |
the recombinant Bombyx mori MTHFD (bmMTHFD) recognizes 5,10-methylenetetrahydrofolate and 5,10-methenyltetrahydrofolate as substrates in the presence of NADP+ as well as NAD+ |
764677 |
1.5.1.5 | NAD+ |
- |
742856, 764677 |
1.5.1.5 | NAD+ |
23% of the activity with NADP+ |
392326 |
1.5.1.5 | NAD+ |
instead of NADP as in cytosolic dehydrogenase precursor (higher Km and lower Vmax with NADP) |
701402 |
1.5.1.5 | NAD+ |
mitochondrial dehydrogenase activity |
701402 |
1.5.1.5 | NAD+ |
NADP+ preferred cofactor in vivo |
209738 |
1.5.1.5 | NAD+ |
prefers NADP+ as a cofactor, rather than NAD+ |
688323 |
1.5.1.5 | NAD+ |
the enzyme exhibits dual redox cofactor specificity, utilizing either NADP+ or NAD+. NAD+-dependent dehydrogenase activity of MTHFD2 with the substrate 5,10-methylenetetrahydropteroylglutamate is 8.5fold higher than its NADP+-dependent activity. While the NAD+-dependent activity of MTHFD2 slightly decreases, its maximal NADP+-dependent activity considerably increases with 5,10-methylenetetrahydropteroylpentaglutamate compared to 5,10-methylenetetrahydropteroylglutamate |
756334 |