EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    6.3.3.2 | -999 |
- |
more |
- |
1319, 1320, 1321, 1328 |
| 6.3.3.2 | 0.02 |
- |
5-formyltetrahydrofolate |
mutant enzyme Y152A, in 100 mM MES, pH 6.0, at 37°C |
671619 |
| 6.3.3.2 | 0.046 |
- |
5-formyltetrahydrofolate |
mutant enzyme R7A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C |
728751 |
| 6.3.3.2 | 0.1 |
- |
5-formyltetrahydrofolate |
mutant enzyme K3D/D151K, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C |
728751 |
| 6.3.3.2 | 0.17 |
- |
5-formyltetrahydrofolate |
mutant enzyme K3A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C |
728751 |
| 6.3.3.2 | 0.35 |
- |
5-formyltetrahydrofolate |
mutant enzyme R125A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C |
728751 |
| 6.3.3.2 | 0.77 |
- |
5-formyltetrahydrofolate |
mutant enzyme D81A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C |
728751 |
| 6.3.3.2 | 0.79 |
- |
5-formyltetrahydrofolate |
wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C |
694983 |
| 6.3.3.2 | 0.8 |
- |
5-formyltetrahydrofolate |
mutant enzyme W153A, in 200 mM MES (pH 6.0), 30 mM sodium chloride, 10 mM magnesium chloride, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol at 37°C |
728751 |
| 6.3.3.2 | 0.92 |
- |
5-formyltetrahydrofolate |
mutant enzyme Y151A, in 100 mM MES, pH 6.0, at 37°C |
671619 |
