EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
6.1.1.27 | -999 |
- |
more |
turnover number for tRNACys isoacceptor |
687743 |
6.1.1.27 | 0.0054 |
- |
O-phospho-L-threonine |
wild-type enzyme |
693106 |
6.1.1.27 | 0.008 |
- |
tRNACys |
pH 7.6, 50°C, mutant D418N/D420N/T423V |
690738 |
6.1.1.27 | 0.014 |
- |
O-phospho-L-threonine |
pH 7.5, 37°C, recombinant enzyme |
693106 |
6.1.1.27 | 0.021 |
- |
O-phospho-L-threonine |
pH 7.5, 37°C, recombinant mutant T307S |
693106 |
6.1.1.27 | 0.0471 |
- |
tRNACys |
50°C, pH 7.6, tRNACys containing the (pyrrole-2-carbaldehyde)UA anticodon, mutant enzyme D418N D420N T423V |
690738 |
6.1.1.27 | 0.048 |
- |
tRNACys |
50°C, pH 7.6, tRNACys containing the C(pyrrole-2-carbaldehyde)A anticodon, mutant enzyme D418N D420N T423V |
690738 |
6.1.1.27 | 0.07 |
- |
tRNACys |
60°C, pH 6.0, steady-state kinetics |
689145 |
6.1.1.27 | 0.07 |
- |
tRNACys |
at pH 7.5 and 60°C |
746291 |
6.1.1.27 | 0.115 |
- |
tRNACys |
50°C, pH 7.6, tRNACys with the GCA anticodon, wild-type enzyme |
690738 |