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EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Reference
6.1.1.21	-999	-	more	-	373
6.1.1.21	0.0004	-	tRNAHis	mutant E83A, pH 7.5, 37°C	661192
6.1.1.21	0.0006	-	tRNAHis	mutant R159H, pH 7.5, 37°C	661192
6.1.1.21	0.0013	-	RNA microhelix	pH 8.0, 37°C, aminoacylation	677092
6.1.1.21	0.002	-	synthetic tRNAHis A-1	7.5, 37°C, enzyme mutant R123A	650256
6.1.1.21	0.005	-	U73tRNAHisGUG	full-length HisRS, aminoacylation	361
6.1.1.21	0.01	-	tRNAHis	mutant E83Q, pH 7.5, 37°C	661192
6.1.1.21	0.012	-	synthetic tRNAHis A-1	7.5, 37°C, enzyme mutant R116A	650256
6.1.1.21	0.016	-	U73tRNAHisGUG	truncated enzyme form NcatHisRS, aminoacylation	361
6.1.1.21	0.018	-	synthetic tRNAHis A-1	7.5, 37°C, wild-type enzyme	650256

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Release 2023.1 (January 2023)