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EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Reference
5.4.99.2
0.003
-
(R)-2-methylmalonyl-CoA
37°C, mutant H610N
652252
5.4.99.2
0.012
-
(R)-methylmalonyl-CoA
recombinant His-tagged mutant E392D, pH 8.5, 25°C
727036
5.4.99.2
0.024
-
(R)-2-methylmalonyl-CoA
37°C, mutant H610A
652252
5.4.99.2
0.038
-
succinyl-CoA
25°C, mutant H244A
650005
5.4.99.2
0.25
-
(R)-methylmalonyl-CoA
recombinant His-tagged mutant E392Q, pH 8.5, 25°C
727036
5.4.99.2
0.32
-
(R)-methylmalonyl-CoA
recombinant His-tagged mutant E392A, pH 8.5, 25°C
727036
5.4.99.2
0.33
-
(R)-2-methylmalonyl-CoA
mutant Y243A, pH 7.0, 37°C
678179
5.4.99.2
0.66
-
succinyl-CoA
25°C, mutant H244Q
650005
5.4.99.2
3.95
-
(R)-methylmalonyl-CoA
recombinant His-tagged wild-type enzyme, pH 8.5, 25°C
727036
5.4.99.2
6.08
-
succinyl-CoA
25°C, mutant H244Q
650005
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