EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
5.4.99.1 | 0.0181 |
- |
L-glutamate |
25°C, mutant enzyme R100Y |
650247 |
5.4.99.1 | 0.0211 |
- |
L-glutamate |
25°C, mutant enzyme R100M |
650247 |
5.4.99.1 | 0.025 |
- |
L-glutamate |
23°C, mutant enzyme E171N |
650824 |
5.4.99.1 | 0.048 |
- |
L-glutamate |
25°C, mutant enzyme R100K |
650247 |
5.4.99.1 | 0.05 |
- |
L-2-hydroxyglutarate |
pH 7.0 |
649913 |
5.4.99.1 | 0.073 |
- |
L-threo-3-methylaspartate |
mutant R100K, pH 7.0 |
677821 |
5.4.99.1 | 0.11 |
- |
L-glutamate |
23°C, mutant enzyme E171Q |
650824 |
5.4.99.1 | 0.21 |
- |
L-glutamate |
23°C, mutant enzyme E171A |
650824 |
5.4.99.1 | 0.65 |
- |
L-Glu |
reaction of L-Glu with benzimidazolribofuranosyl-adenosylcobinamide, fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit |
3458 |
5.4.99.1 | 3 |
6 |
L-glutamate |
23°C, mutant enzyme E171D |
650824 |