EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
5.3.2.1 | 0.0004 |
- |
trans-3-chloroacrylate |
wild type enzyme CCH2, in in 50 mM Tris-SO4 buffer (pH 8.0), at 22°C |
714241 |
5.3.2.1 | 0.03 |
- |
keto-(p-hydroxyphenyl)pyruvate |
25°C, pH 6.0, mutant enzyme P1(A)M2 |
649900 |
5.3.2.1 | 0.052 |
- |
(4-hydroxyphenyl)pyruvate |
enol-form, kidney |
2872 |
5.3.2.1 | 0.2 |
- |
(4-hydroxyphenyl)enolpyruvate |
wild type enzyme CCH2-P1A, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C |
714241 |
5.3.2.1 | 0.2 |
- |
phenylenolpyruvate |
wild type enzyme cis-CAAD, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C |
714241 |
5.3.2.1 | 0.45 |
- |
phenylpyruvate |
keto-form, kidney |
2872 |
5.3.2.1 | 0.6 |
- |
enol-phenylpyruvate |
23°C, mutant enzyme P1G |
650013 |
5.3.2.1 | 0.8 |
- |
enol-(p-hydroxyphenyl)pyruvate |
23°C, mutant enzyme P1G |
650013 |
5.3.2.1 | 0.8 |
- |
phenylenolpyruvate |
mutant enzyme CCH2-P1A, in 10 mM Na2HPO4 buffer, pH 7.3, at 22°C |
714241 |
5.3.2.1 | 0.93 |
- |
keto-(p-hydroxyphenyl)pyruvate |
25°C, pH 6.0, mutant enzyme P1G |
649900 |