EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    5.1.1.3 | -999 |
- |
more |
mutant V149A has about a 2fold higher kcat than wild-type RacE2 (67/sec versus 38/sec). In the reverse reaction, V149A has a higher kcat than RacE2 (4.9/sec versus 1.6/sec, respectively) |
675451 |
| 5.1.1.3 | 0.00034 |
- |
D-glutamate |
pH 8.5, mutant enzyme D7S |
653305 |
| 5.1.1.3 | 0.0023 |
- |
L-glutamate |
pH 8.5, mutant enzyme E147N |
653305 |
| 5.1.1.3 | 0.0047 |
- |
L-glutamate |
pH 8.5, mutant enzyme E147N |
653305 |
| 5.1.1.3 | 0.017 |
- |
L-glutamate |
recombinant His-tagged enzyme, pH 8.0, 37°C |
759787 |
| 5.1.1.3 | 0.024 |
- |
L-glutamate |
recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer |
759787 |
| 5.1.1.3 | 0.025 |
- |
D-glutamate |
recombinant enzyme, pH 8.0, 37°C |
749098 |
| 5.1.1.3 | 0.037 |
- |
L-glutamate |
pH 8.5, mutant enzyme D7S |
653305 |
| 5.1.1.3 | 0.045 |
- |
L-glutamate |
pH 8, 30°C, mutant enzyme H186N |
650067 |
| 5.1.1.3 | 0.056 |
- |
D-glutamate |
pH 8.5, wild-type enzyme |
653305 |
