EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
4.3.2.10 | -999 |
- |
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide |
pH 7.0, 25°C, kinetic constants are determined for wild-type and mutant enzymes |
747285 |
4.3.2.10 | -999 |
- |
L-glutamine |
pH 7.0, 25°C, kinetic constants are determined for wild-type and mutant enzymes |
747285 |
4.3.2.10 | 0.00165 |
- |
L-glutamine |
pH 8.0, 37°C, wild-type enzyme |
749186 |
4.3.2.10 | 0.00173 |
- |
L-glutamine |
pH 8.0, 37°C, HisF mutant enzyme V12A |
749186 |
4.3.2.10 | 0.00311 |
- |
L-glutamine |
pH 8.0, 37°C, mutant enzyme D98A |
749186 |
4.3.2.10 | 0.00328 |
- |
L-glutamine |
pH 8.0, 37°C, mutant enzyme V48A |
749186 |
4.3.2.10 | 0.00372 |
- |
L-glutamine |
pH 8.0, 30°C |
747861 |
4.3.2.10 | 0.00372 |
- |
L-glutamine |
pH 8.0, 30°C, basal activity |
747861 |
4.3.2.10 | 0.0054 |
- |
L-glutamine |
pH 8.0, 37°C, HisF mutant enzyme K19A |
749186 |
4.3.2.10 | 0.045 |
- |
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide |
30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258A |
747058 |