EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   4.2.99.B1 | -999 |
- |
intact apurinic/apyrimidinic site |
200fold lower compared to preincised apurinic/apyrimidinic site as substrate |
692941 |
| 4.2.99.B1 | 0.0018 |
- |
28-mer deoxyoligonucleotide with a 5' uracil residue |
pretreated with Eschericia coli uracil N-glycosylase, with concentrations 0.1-2.0 microMol, kcat/Km: 0.24 microMol/min |
692962 |
| 4.2.99.B1 | 0.0043 |
- |
preincised apurinic/apyrimidinic DNA |
pH 7.4, 37°C, in presence of 0.2 mM EDTA |
694925 |
| 4.2.99.B1 | 0.007 |
- |
35-bp-deoxyoligonucleotide duplex containing a uracil residue at position 15 |
full-length 290-kDa Pol theta, pH 7.5, 37°C, kcat/Km: 0.047 microM/min |
694467 |
| 4.2.99.B1 | 0.0075 |
- |
DNA with a 5'-incised apurinic/apyrimidinic site at position 21 |
dRP lyase activity of beta-pol. To quantify the kinetic parameters for the release of deoxyribose phosphate from a preincused apurinic/apyrimidinic site-containing DNA, deoxyribose phosphate is examined as a function of apurinic/apyrimidinic site concentration. The catalytic efficiency results in a kcat/Km: 0.00015 mM/s. The catalytic efficiency of the 8-kDa amino-terminal domain is 7fold lower than that of the intact 39-kDa beta-pol |
692941 |
| 4.2.99.B1 | 0.0075 |
- |
mimic of a preincised DNA duplex with a 5'dRP at the site of the nick |
pH 7.5, 37°C |
694866 |
| 4.2.99.B1 | 0.01167 |
- |
35-bp-deoxyoligonucleotide duplex containing a uracil residue at position 15 |
98-kDa fragment of Pol theta, pH 7.5, 37°C, kcat/Km: 0.072 microM/min |
694467 |
| 4.2.99.B1 | 4.5 |
- |
preincised apurinic/apyrimidinic DNA |
recombinant protein, pH 7.4, 37°C, in presence of 0.2 mM EDTA |
694925 |
