EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
4.2.1.114 | 0.37 |
- |
(R)-homocitrate |
pH 8.5, 60°C, recombinant enzyme, dehydratase reaction |
693150 |
4.2.1.114 | 0.48 |
- |
but-1-ene-1,2,4-tricarboxylic acid |
mutant enzyme R26V |
702368 |
4.2.1.114 | 0.66 |
- |
(1E)-pent-1-ene-1,2,5-tricarboxylic acid |
wild type enzyme |
702368 |
4.2.1.114 | 0.66 |
- |
cis-homo2aconitate |
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction |
693150 |
4.2.1.114 | 0.75 |
- |
but-1-ene-1,2,4-tricarboxylic acid |
wild type enzyme |
702368 |
4.2.1.114 | 0.75 |
- |
cis-homoaconitate |
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction |
693150 |
4.2.1.114 | 1.43 |
- |
but-1-ene-1,2,4-tricarboxylic acid |
mutant enzyme R26K |
702368 |
4.2.1.114 | 1.7 |
- |
but-1-ene-1,2,4-tricarboxylic acid |
mutant enzyme R26V/T27Y |
702368 |
4.2.1.114 | 1.9 |
- |
(1E)-hex-1-ene-1,2,6-tricarboxylic acid |
mutant enzyme R26V/T27Y |
702368 |
4.2.1.114 | 2.2 |
- |
(1E)-pent-1-ene-1,2,5-tricarboxylic acid |
mutant enzyme T27A |
702368 |