EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   4.1.1.82 | 0.31 |
- |
3-phosphonopyruvate |
mutant enzyme S25D, at pH 8.0 (HEPES buffer) and at 30°C |
705200 |
| 4.1.1.82 | 0.55 |
- |
3-phosphonopyruvate |
mutant enzyme D297N, at pH 8.0 (HEPES buffer) and at 30°C |
705200 |
| 4.1.1.82 | 0.76 |
- |
3-phosphonopyruvate |
mutant enzyme D297E, at pH 8.0 (HEPES buffer) and at 30°C |
705200 |
| 4.1.1.82 | 0.78 |
- |
3-phosphonopyruvate |
mutant enzyme S25N, at pH 8.0 (HEPES buffer) and at 30°C |
705200 |
| 4.1.1.82 | 1.18 |
- |
3-phosphonopyruvate |
wild type enzyme, at pH 8.0 (HEPES buffer) and at 30°C |
705200 |
| 4.1.1.82 | 1.24 |
- |
3-phosphonopyruvate |
mutant enzyme H111A, at pH 8.0 (HEPES buffer) and at 30°C |
705200 |
| 4.1.1.82 | 10.1 |
- |
3-phosphonopyruvate |
pH 7.0, 30°C |
747261 |
| 4.1.1.82 | 10.2 |
- |
3-phosphonopyruvate |
25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine diphosphate |
659267 |
