EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    4.1.1.49 | -999 |
- |
more |
kcat for oxalacetate as a substrate is above 19 1/s for S252A mutant in the phosphoenolpyruvate formation direction |
691133 |
| 4.1.1.49 | 0.014 |
- |
phosphoenolpyruvate |
mutant enzyme K212R, in 100 mM MOPS buffer (pH 6.6), at 30°C |
702488 |
| 4.1.1.49 | 0.07 |
- |
phosphoenolpyruvate |
mutant enzyme E284Q, in 100 mM MOPS buffer (pH 6.6), at 30°C |
702488 |
| 4.1.1.49 | 0.08 |
- |
oxaloacetate |
Km above 2.0 mM, mutant enzyme E284Q, in 100 mM MOPS buffer (pH 6.6), at 30°C |
702488 |
| 4.1.1.49 | 0.08 |
- |
oxaloacetate |
mutant enzyme E272Q, in 100 mM MOPS buffer (pH 6.6), at 30°C |
702488 |
| 4.1.1.49 | 0.11 |
- |
oxaloacetate |
mutant enzyme K212R, in 100 mM MOPS buffer (pH 6.6), at 30°C |
702488 |
| 4.1.1.49 | 0.13 |
- |
phosphoenolpyruvate |
mutant enzyme K212M, in 100 mM MOPS buffer (pH 6.6), at 30°C |
702488 |
| 4.1.1.49 | 0.2 |
- |
phosphoenolpyruvate |
for the pyruvate kinase-like activity of S252A mutant |
691133 |
| 4.1.1.49 | 0.28 |
- |
oxaloacetate |
wild type enzyme, in 100 mM MOPS buffer (pH 6.6), at 30°C |
702488 |
| 4.1.1.49 | 0.32 |
- |
oxalacetate |
for the oxaloacetate decarboxylase activity of wild-type |
691133 |
