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EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Reference
4.1.1.19
-999
-
more
-
4019
4.1.1.19
-999
-
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To ascertain whether the ADC enzyme expressed in transgenic Trypanosoma cruzi has a modified metabolic turnover when attached to the His-tag, cycloheximide is added to parasite cultures transformed with plasmids to stop protein synthesis. ADC half-life inside the parasite is 150 min, the enzyme attached to the His-tail is more stable, with a half-life of about 14 h.
692241
4.1.1.19
0.025
-
L-arginine
at pH 7.5, 60°C
715563
4.1.1.19
0.04
-
L-ornithine
at pH 7.5, 70°C
715563
4.1.1.19
0.11
-
L-canavanine
pH 7.5, 37°C
654689
4.1.1.19
0.21
-
L-arginine
at pH 7.5, 37°C
715563
4.1.1.19
0.419
-
L-N5-(iminoethyl)-ornithine
pH 7.5, 37°C
654689
4.1.1.19
0.48
-
L-arginine
at pH 8.5, 30°C
715563
4.1.1.19
0.9
-
L-arginine
untagged ADC
692241
4.1.1.19
0.92
-
L-arginine
pH 7.4, 37°C
747997
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