EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    3.7.1.3 | 0.001 |
- |
beta-(4-trifluoromethylbenzoyl)-DL-alanine |
at 37°C in 0.04 M potassium phosphate, pH 7.8 |
718881 |
| 3.7.1.3 | 0.0039 |
- |
3-hydroxy-L-kynurenine |
kcat less than 0.0039 1/s, mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C |
699466 |
| 3.7.1.3 | 0.0058 |
- |
L-kynurenine |
enzyme mutant Y226F, pH 8.0, 37°C |
733832 |
| 3.7.1.3 | 0.0058 |
- |
L-kynurenine |
pH 8.0, 37°C, mutant enzyme Y226F |
756117 |
| 3.7.1.3 | 0.007 |
- |
L-kynurenine |
mutant enzyme H102W/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C |
699466 |
| 3.7.1.3 | 0.0109 |
- |
3-hydroxy-L-kynurenine |
kcat less than 0.0109 1/s, mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C |
699466 |
| 3.7.1.3 | 0.017 |
- |
L-kynurenine |
mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C |
699466 |
| 3.7.1.3 | 0.018 |
- |
L-kynurenine |
Km above 0.51 mM, mutant enzyme H102W/S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C |
699466 |
| 3.7.1.3 | 0.026 |
- |
L-kynurenine |
mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37°C |
699466 |
| 3.7.1.3 | 0.05 |
- |
2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid |
at 37°C in 0.04 M potassium phosphate, pH 7.8 |
718881 |
