EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.6.1.39 | 0.05 |
- |
dTTP |
mutant enzyme N468A, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.05 |
- |
dTTP |
mutant enzyme R493A/N468A, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.06 |
- |
dTTP |
mutant enzyme N468A, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.08 |
- |
dTTP |
mutant enzyme R493Q, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.22 |
- |
dTTP |
mutant enzyme R493Q, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.22 |
- |
dTTP |
wild type enzyme, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.35 |
- |
dTTP |
mutant enzyme R493A/N468A, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 20 |
- |
dTTP |
wild type enzyme, in the presence of M13 single-stranded DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |