EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.4.11.4 | -999 |
- |
more |
- |
653227 |
3.4.11.4 | 0.04 |
1.97 |
Ala-Ala-Phe-7-amido-4-methylcoumarin |
pH 5.0, 37°C, mutant enzyme D276A |
664950 |
3.4.11.4 | 0.12 |
- |
Ala-Ala-Phe-7-amido-4-methylcoumarin |
pH 5.0, 37°C, mutant enzyme S475A |
664950 |
3.4.11.4 | 0.56 |
- |
Arg-Ser-Arg |
calculated value, wild type enzyme |
682786 |
3.4.11.4 | 1.52 |
- |
Ala-Ala-Phe-7-amido-4-methylcoumarin |
pH 5.0, 37°C, mutant enzyme D360A |
664950 |
3.4.11.4 | 1.54 |
- |
Ala-Ala-Phe-7-amido-4-methylcoumarin |
pH 5.0, 37°C, mutant enzyme E272A |
664950 |
3.4.11.4 | 3.22 |
- |
Ala-Ala-Phe-7-amido-4-methylcoumarin |
pH 5.0, 37°C, wild-type enzyme |
664950 |
3.4.11.4 | 4.22 |
- |
Ala-Ala-Phe-7-amido-4-methylcoumarin |
pH 5.0, 37°C, mutant enzyme D327A |
664950 |
3.4.11.4 | 5.3 |
- |
Arg-Ala-Arg |
calculated value, wild type enzyme |
682786 |
3.4.11.4 | 6.8 |
- |
Ala-Phe-Pro-beta-naphthylamide |
mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C |
681470 |