EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.2.1.25 | -999 |
- |
more |
as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the turnover number of 167 per s is only an estimate |
654244 |
| 3.2.1.25 | 0.00183 |
- |
2,4-dinitrophenyl-beta-D-mannopyranoside |
mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.008 |
- |
2,4-dinitrophenyl-beta-D-mannopyranoside |
mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.0097 |
- |
p-nitrophenyl-beta-D-mannopyranoside |
mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.033 |
- |
4-nitrophenyl-beta-D-mannopyranose |
pH 7.0, 37°C, wild-type enzyme |
665524 |
| 3.2.1.25 | 0.045 |
- |
p-nitrophenyl-beta-D-mannopyranoside |
mutant enzyme D199A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.053 |
- |
beta-D-mannosyl-1,4-beta-D-glucoside |
pH 7.0, 37°C, wild-type enzyme |
665524 |
| 3.2.1.25 | 0.092 |
- |
p-nitrophenyl-beta-D-mannopyranoside |
mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.1 |
- |
4-nitrophenyl-beta-mannopyranose |
pH 7.0, 37°C, mutant enzyme E215A |
665524 |
| 3.2.1.25 | 0.25 |
- |
p-nitrophenyl-beta-D-mannopyranoside |
mutant enzyme N461A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
