EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.1.3.32 | -999 |
- |
2',3'-cAMP |
mutant enzyme D236E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C |
682186 |
3.1.3.32 | 0.003 |
- |
phosphorylated DNA ends |
- |
652544 |
3.1.3.32 | 0.0043 |
- |
phosphorylated DNA ends |
- |
652544 |
3.1.3.32 | 0.017 |
- |
cAMP |
- |
659503 |
3.1.3.32 | 0.04 |
1.97 |
2',3'-cAMP |
mutant enzyme R237A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C |
682186 |
3.1.3.32 | 0.04 |
1.97 |
2',3'-cAMP |
wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C |
682186 |
3.1.3.32 | 0.16 |
- |
3'-TMP |
- |
659503 |
3.1.3.32 | 0.26 |
- |
bis-p-nitrophenyl phosphate |
mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 |
674594 |
3.1.3.32 | 0.42 |
- |
3'-phospho-5'-hydroxy-DNA |
at pH 7.5 and 37°C |
730000 |
3.1.3.32 | 0.52 |
- |
bis-p-nitrophenyl phosphate |
mutant enzyme D392N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 |
674594 |