EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.1.13.2 | -999 |
- |
more |
kcat value of Avian myeloblastosis virus reverse transcriptase (RNase H reaction) is above 0.025 s-1. The kcat values for different Moloney murine leukaemia virus/Avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 6.3 1/s for MRT-AF, 3.8 1/s for MRT-AP, 4.6 1/s for MRT-AT, and 2.4 1/s for MRT-AR |
692923 |
3.1.13.2 | -999 |
- |
more |
kcat value of Moloney murine leukemia virus reverse transcriptase (RNase H reaction) above 3 ms-1. The kcat values for different Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptases with poly(rA)*p(dT)45 as a substrate: 6.3 1/s for MRT-AF, 3.8 1/s for MRT-AP, 4.6 1/s for MRT-AT, and 2.4 1/s for MRT-AR |
692923 |
3.1.13.2 | -999 |
- |
more |
study of the order of cleavage, most RNA fragments are cleaved by a combination of primary, secondary primary, and 5-nt cuts within 1 min |
653638 |
3.1.13.2 | 0.0018 |
- |
DNA-17-nucleotide-RNA hybrid |
pH 7.8, 37°C, recombinant wild-type enzyme |
664079 |
3.1.13.2 | 0.0024 |
- |
poly(rA)n-poly(dT)n |
mutant p66/p51C280W |
652907 |
3.1.13.2 | 0.0024 |
- |
poly(rA)n-poly(dT)ne |
mutant p66C280P/p51 |
652907 |
3.1.13.2 | 0.0025 |
- |
poly(rA)n-poly(dT)n |
mutant p66/p51C280P |
652907 |
3.1.13.2 | 0.0029 |
- |
poly(rA)n-poly(dT)n |
mutant p66C280P/p51C280P |
652907 |
3.1.13.2 | 0.0029 |
- |
poly(rA)n-poly(dT)n |
mutant p66C280W/p51 |
652907 |
3.1.13.2 | 0.0031 |
- |
poly(rA)n-poly(dT)n |
wild type enzyme p66/p51 |
652907 |