EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.1.1.56 | -999 |
- |
dimethyl p-nitrophenyl phosphate |
no detectable phosphorylation rate, double mutant W197I/C60S, 22°C |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant C60H/W197I, catalytic efficiency kcat/KM: 260 +/-20 1/mM/min |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant C60K/W197I, catalytic efficiency kcat/KM: 390 +/-30 1/mM/min |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant C60Q/W197I, catalytic efficiency kcat/KM: 320 +/-30 1/mM/min |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant C60R/W197I, catalytic efficiency kcat/KM: 1500 +/-200 1/mM/min |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant C60S, after removal of beta-mercaptoethanol, catalytic efficiency kcat/KM: 300 +/-60 1/mM/min |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant C60S, catalytic efficiency kcat/KM: 480 +/-80 1/mM/min |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant C60S/W197I, catalytic efficiency kcat/KM: 330 +/-20 1/mM/min |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant L58F/W197I, Lineweaver-Burke extrapolation, catalytic efficiency kcat/KM: 1.5 +/-0.5 1/mM/min |
691005 |
3.1.1.56 | -999 |
- |
p-nitrophenyl acetate |
mutant M162H, catalytic efficiency kcat/KM: 7 +/-3 1/mM/min |
691005 |