EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.7.1.23 | -999 |
- |
more |
the molecular activity is 360 per min, the activity per active centre is less than 60 per min, pH 7.4, 30°C |
641182 |
2.7.1.23 | 0.41 |
- |
NAD+ |
mutant D45N, in the presence of 0.5 mM ATP |
693014 |
2.7.1.23 | 0.5 |
- |
NAD+ |
mutant enzyme P57S/P117S, at pH 6.0 and 30°C |
722144 |
2.7.1.23 | 0.52 |
- |
ATP |
wild type enzyme, at pH 7.5 and 30°C |
722144 |
2.7.1.23 | 0.54 |
- |
NAD+ |
wild type enzyme, at pH 7.5 and 30°C |
722144 |
2.7.1.23 | 0.6 |
- |
ATP |
mutant enzyme P57S/P117S, at pH 6.0 and 30°C |
722144 |
2.7.1.23 | 1.34 |
- |
NAD+ |
mutant D45N, in the presence of 4 mM ATP |
693014 |
2.7.1.23 | 1.36 |
- |
ATP |
- |
641186 |
2.7.1.23 | 1.8 |
- |
polyphosphate(75) |
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C |
721366 |
2.7.1.23 | 1.9 |
- |
NAD+ |
in 100 mM Tris-HCl (pH 7.5), at 37°C |
721766 |