EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.1.1.258 | 0.00003 |
- |
methylcobalamin |
mutant enzyme N199A, at pH 8.4 and 55°C |
719846 |
2.1.1.258 | 0.005 |
- |
[Co(I) corrinoid Fe-S protein] |
mutant enzyme N199A, in 0.1 M potassium succinate, pH 5.0, 60 mM NaCl, at 25°C |
719846 |
2.1.1.258 | 0.02 |
- |
[methyl-Co(III) corrinoid Fe-S protein] |
kcat below 0.02 s(-1), in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
2.1.1.258 | 0.05 |
- |
methylcobalamin |
wild type enzyme, at pH 8.4 and 55°C |
719846 |
2.1.1.258 | 1.1 |
- |
hydroxocobalamin |
in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
2.1.1.258 | 1.1 |
- |
[Co(I) corrinoid Fe-S protein] |
wild type enzyme, in 0.1 M potassium succinate, pH 5.0, 60 mM NaCl, at 25°C |
719846 |
2.1.1.258 | 1.5 |
- |
5-methyltetrahydrofolate |
using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
2.1.1.258 | 2.2 |
- |
methylcobalamin |
in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
2.1.1.258 | 2.63 |
- |
Cobalamin |
25°C, pH 5.1 |
728242 |
2.1.1.258 | 14.7 |
- |
5-methyltetrahydrofolate |
in 50 mM MES, pH 5.1, at 25°C |
717236 |