EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    1.5.1.30 | -999 |
- |
more |
coenzyme clamps formed by arginine side chains at two co-evolving positions within the active site serve to slow coenzyme release (Positions 14 and 78). Coenzyme release is further slowed by the weaker binding substrate, resulting in relatively slow turnover numbers |
765021 |
| 1.5.1.30 | 0.0282 |
- |
NADPH |
with FMN, pH 7.5, 30°C, recombinant enzyme |
743224 |
| 1.5.1.30 | 0.036 |
- |
NADPH |
with FAD, pH 7.5, 30°C, recombinant enzyme |
743224 |
| 1.5.1.30 | 0.061 |
- |
FAD |
pH and temperature not specified in the publication |
765021 |
| 1.5.1.30 | 0.099 |
- |
FMN |
pH 7.5, 25°C |
714077 |
| 1.5.1.30 | 0.3 |
- |
FAD |
pH 7.5, 25°C |
714077 |
| 1.5.1.30 | 0.3 |
- |
oxidized riboflavin |
pH 7.5, 25°C |
714077 |
| 1.5.1.30 | 0.7 |
- |
Cibacron Marine |
pH 7.5, 37°C |
672184 |
| 1.5.1.30 | 1.7 |
- |
FMN |
25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate |
657786 |
| 1.5.1.30 | 1.9 |
- |
FMN |
25°C, single-enzyme assay |
657786 |
