EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.2.4.2 | 0.0595 |
- |
2-oxo-5-hexenoic acid |
wild type enzyme, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.148 |
- |
2-oxo-5-hexenoic acid |
mutant enzyme H298D, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.152 |
- |
2-oxovalerate |
wild type enzyme, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.34 |
- |
2-oxovalerate |
mutant E1o-H298D/E2o-H348Q, pH not specified in the publication, temperature not specified in the publication |
762591 |
1.2.4.2 | 0.38 |
- |
2-oxovalerate |
mutant E1o-H298D/E2o-S333M, pH not specified in the publication, temperature not specified in the publication |
762591 |
1.2.4.2 | 0.48 |
- |
2-oxovalerate |
mutant enzyme H298D, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.57 |
- |
2-oxovalerate |
mutant E1o-H298D/E2o-H348Y, pH not specified in the publication, temperature not specified in the publication |
762591 |
1.2.4.2 | 0.65 |
- |
2-oxo-5-hexenoic acid |
mutant E1o-H298D/E2o-H348Q, pH not specified in the publication, temperature not specified in the publication |
762591 |
1.2.4.2 | 0.73 |
- |
2-oxoglutarate |
mutant enzyme H298D, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.81 |
- |
2-oxovalerate |
mutant E1o-H298D/E2o-H348F, pH not specified in the publication, temperature not specified in the publication |
762591 |