EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   1.14.13.39 | 0.033 |
- |
nitric oxide |
DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C |
698852 |
| 1.14.13.39 | 0.055 |
- |
nitric oxide |
DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C |
698852 |
| 1.14.13.39 | 0.28 |
- |
NADPH |
DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C |
698852 |
| 1.14.13.39 | 0.32 |
- |
NADPH |
DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C |
698852 |
| 1.14.13.39 | 0.62 |
- |
mitomycin C |
full-length enzyme, absence of calmodulin |
657670 |
| 1.14.13.39 | 0.65 |
- |
nitroblue tetrazolium |
NADPH-diaphorase activity |
440196 |
| 1.14.13.39 | 0.8 |
- |
mitomycin C |
FAD-NADPH domain |
657670 |
| 1.14.13.39 | 0.92 |
- |
nitric oxide |
wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C |
698852 |
| 1.14.13.39 | 1.08 |
- |
L-arginine |
- |
440196 |
| 1.14.13.39 | 1.23 |
- |
nitric oxide |
wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C |
698852 |
