EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    1.1.2.3 | -999 |
- |
more |
second-order rate constant for cytochrome c reduction in the pre-steady-state determined by stopped-flow spectrophotometry |
696188 |
| 1.1.2.3 | -999 |
- |
more |
steady-state kinetic parameters and 2H kinetic isotope effects for the isolated flavin domain and intact, wild-type flavocytoochrome b2 |
696075 |
| 1.1.2.3 | -999 |
- |
more |
systematic determination of activity of all mutants with L-mandelate and L-lactate as substrate |
348075 |
| 1.1.2.3 | -999 |
- |
more |
systematic determination of substrates with different chain length |
348070 |
| 1.1.2.3 | -999 |
- |
more |
systematic determination of turnover numbers for wild type enzyme and deletion mutants with different electron acceptors, significantly reduced activity for deletion mutants with cytochrome c as electron acceptor |
348073 |
| 1.1.2.3 | 0.011 |
- |
ferricytochrome c |
pH 7.5, 25°C, Tris-HCl buffer, mutant Y143F |
722153 |
| 1.1.2.3 | 0.02 |
- |
ferricytochrome c |
pH 7.5, 25°C, recombinantly expressed flavocytochrome b2 flavin-binding domain |
696075 |
| 1.1.2.3 | 0.02 |
- |
L-Mandelate |
wild type enzyme with ferricyanide as electron acceptor |
348075 |
| 1.1.2.3 | 0.031 |
- |
L-lactate |
mutant H373Q, intact protein |
685174 |
| 1.1.2.3 | 0.057 |
- |
L-lactate |
mutant H373Q, using flavin domain only |
685174 |
