EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.1.B4 | 0.04 |
- |
3'-trifluoromethyl-acetophenone |
pH 6.5, 50°C, wild-type enzyme |
741784 |
1.1.1.B4 | 0.08 |
- |
3'-methoxy-acetophenone |
pH 6.5, 50°C, wild-type enzyme |
741784 |
1.1.1.B4 | 0.18 |
- |
2'-methylacetophenone |
pH 6.5, 50°C, wild-type enzyme |
741784 |
1.1.1.B4 | 0.47 |
- |
1-(3-pyridyl)acetone |
pH 6.5, 50°C, mutant I86A |
741784 |
1.1.1.B4 | 0.61 |
- |
3'-iodo-acetophenone |
pH 6.5, 50°C, wild-type enzyme |
741784 |
1.1.1.B4 | 0.66 |
- |
3'-chloro-acetophenone |
pH 6.5, 50°C, mutant I86A |
741784 |
1.1.1.B4 | 0.84 |
- |
NAD+ |
pH 6.0, 65°C, recombinant enzyme, reduction reaction |
684583 |
1.1.1.B4 | 1 |
- |
(R)-2-butanol |
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C |
697839 |
1.1.1.B4 | 1.01 |
- |
acetone |
pH 6.5, 50°C, mutant I86A |
741784 |
1.1.1.B4 | 1.1 |
- |
(R)-2-pentanol |
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C |
697839 |