EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.1.93 | -999 |
- |
more |
D-malate oxidation is largely limited by the rate of decarboxylation of the intermediate oxaloacetate which occurs at 660 per min. Hydride transfer from D-malate to NAD+ occurs with a rate constant of 1800 per min |
389614 |
1.1.1.93 | 0.015 |
- |
NAD+ |
mutant D225A, pH 7.5, temperature not specified in the publication |
710720 |
1.1.1.93 | 0.06 |
- |
NAD+ |
mutant R108Q, pH 7.5, temperature not specified in the publication |
710720 |
1.1.1.93 | 0.087 |
- |
NAD+ |
mutant R108L, pH 7.5, temperature not specified in the publication |
710720 |
1.1.1.93 | 0.088 |
- |
NAD+ |
mutant D250A, pH 7.5, temperature not specified in the publication |
710720 |
1.1.1.93 | 0.167 |
- |
isopropylmalate |
- |
389617 |
1.1.1.93 | 0.175 |
- |
NAD+ |
mutant R98Q, pH 7.5, temperature not specified in the publication |
710720 |
1.1.1.93 | 0.2 |
- |
isopropylmalate |
- |
389612 |
1.1.1.93 | 0.417 |
- |
L-Tartrate |
- |
389617 |
1.1.1.93 | 0.483 |
- |
L-Tartrate |
- |
389612 |