EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.1.86 | 0.0000933 |
- |
NADPH |
pH 8.0, 22°C, reaction with acetolactate, mutant enzyme K75Q |
639183 |
1.1.1.86 | 0.00015 |
- |
NADP+ |
pH 8.0, 22°C, reaction with 2,3-dihydroxy-3-methylbutanoate, mutant enzyme R68Q |
639183 |
1.1.1.86 | 0.0002 |
- |
NADP+ |
pH 8.0, 22°C, reaction with 2,3-dihydroxy-3-methylbutanoate, mutant enzyme R76Q |
639183 |
1.1.1.86 | 0.00023 |
- |
(2S)-2-acetolactate |
pH 8.0, 25°C |
762924 |
1.1.1.86 | 0.00023 |
- |
(S)-2-acetolactate |
pH 8.0, 25°C |
762924 |
1.1.1.86 | 0.000317 |
- |
NADP+ |
pH 8.0, 22°C, reaction with 2,3-dihydroxy-3-methylbutanoate, mutant enzyme K69L |
639183 |
1.1.1.86 | 0.000667 |
- |
NADP+ |
pH 8.0, 22°C, reaction with 2,3-dihydroxy-3-methylbutanoate, mutant enzyme K75Q |
639183 |
1.1.1.86 | 0.00183 |
- |
NADH |
wild type enzyme |
639183 |
1.1.1.86 | 0.0027 |
- |
NADH |
pH 8.0, 22°C |
763172 |
1.1.1.86 | 0.004 |
- |
NADPH |
mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication |
739528 |