EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.1.179 | 0.4 |
- |
D-xylose |
oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose |
684150 |
1.1.1.179 | 0.45 |
- |
D-xylose |
oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose |
684150 |
1.1.1.179 | 0.5 |
- |
D-xylose |
oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose |
684150 |
1.1.1.179 | 0.9 |
- |
D-xylose |
oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose |
684150 |
1.1.1.179 | 1 |
- |
D-xylose |
oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose |
684150 |
1.1.1.179 | 1.2 |
- |
D-xylose |
oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose |
684150 |
1.1.1.179 | 1.5 |
- |
NAD+ |
28°C, pH 7.8 |
723749 |
1.1.1.179 | 1.7 |
- |
D-xylose |
oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose |
684150 |
1.1.1.179 | 2.6 |
- |
D-xylose |
oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose |
684150 |
1.1.1.179 | 5.7 |
- |
D-glucose |
pH 8.0, 30°C, recombinant enzyme |
686797 |