EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s]  |
Substrate |
Reference |
|---|
   6.5.1.3 | -999 |
- |
more |
the kinetic mechanism of single-turnover nick sealing by T4 Rnl2-AMP is explored by using a rapid mix-quench method and the effects of 3'-OH mispairs and base damage lesions on the rates of nick 5'-adenylylation and phosphodiester synthesis is determined. With respect to the sealing of perfectly paired nicks the rates of step 2 catalysis are rapid (9.5-17.9/sec) and similar in magnitude to the step 3 rates (7.9-32/sec). Rnl2 is kinetically sensitive to all 3'-OH base mispairs |
728789 |
| 6.5.1.3 | 0.017 |
- |
GTP |
pH 7.4, 37°C |
651051 |
| 6.5.1.3 | 0.028 |
- |
etidronate |
- |
673669 |
| 6.5.1.3 | 0.05 |
- |
(RNA)n |
pH 6.0, 22°C, multiple-turnover derivatives 210t and 207t, engineered by changing the arrangement of the base pairs in the P2 stem |
649963 |
| 6.5.1.3 | 0.055 |
- |
ATP |
pH 7.4, 30°C, ATP as acceptor of AMP, Ap4A synthesis |
651051 |
| 6.5.1.3 | 0.266 |
- |
(RNA)n |
pH 8.0, 22°C, multiple-turnover derivative 207t, engineered by changing the arrangement of the base pairs in the P2 stem |
649963 |
| 6.5.1.3 | 0.28 |
- |
methylenebisphosphonate |
- |
673669 |
| 6.5.1.3 | 0.29 |
- |
clodronate |
- |
673669 |
| 6.5.1.3 | 0.583 |
- |
(RNA)n |
pH 9.0, 22°C, multiple-turnover derivative 207t, engineered by changing the arrangement of the base pairs in the P2 stem |
649963 |
| 6.5.1.3 | 0.9 |
- |
isopentenyl diphosphate |
- |
707449 |
