EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
6.3.5.7 | -999 |
- |
more |
aminoacylation kinetics of enzyme variants |
728668 |
6.3.5.7 | 0.006 |
- |
tRNAGln1(UUG) |
pH 7.2, 37°C |
728668 |
6.3.5.7 | 0.027 |
- |
L-aspartyl-tRNAAsn |
37°C, pH 7.2, amidotransferase activity |
674787 |
6.3.5.7 | 0.04 |
- |
tRNAGlu |
pH 7.2, 37°C |
728668 |
6.3.5.7 | 0.21 |
- |
Glu-tRNAGln |
in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C |
728391 |
6.3.5.7 | 0.21 |
- |
Glu-tRNAGln |
in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C |
728391 |
6.3.5.7 | 0.23 |
- |
Glu-tRNAGln |
in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C |
728391 |
6.3.5.7 | 0.41 |
- |
tRNAGln2(CUG) |
pH 7.2, 37°C |
728668 |
6.3.5.7 | 0.51 |
- |
Gln |
- |
492302 |
6.3.5.7 | 0.59 |
- |
ATP |
- |
492302 |