EC Number   |
Turnover Number Minimum [1/s]  |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    4.1.3.3 | 0.0002 |
- |
N-acetyl-L-neuraminic acid |
pH 7.5, 25°C, wild-type enzyme |
666750 |
| 4.1.3.3 | 0.0045 |
- |
N-acetyl-L-neuraminic acid |
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I |
666750 |
| 4.1.3.3 | 0.0068 |
- |
N-acetyl-L-neuraminic acid |
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I |
666750 |
| 4.1.3.3 | 0.013 |
- |
N-acetylneuraminate |
mutant Y137F enzyme, pH 7.4, 30°C |
726551 |
| 4.1.3.3 | 0.013 |
- |
N-acetylneuraminate |
purified recombinant mutant K165C, pH 7.4, 30°C |
727317 |
| 4.1.3.3 | 0.015 |
- |
N-acetylneuraminate |
purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C |
727317 |
| 4.1.3.3 | 0.02 |
- |
N-acetyl-L-neuraminic acid |
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y |
666750 |
| 4.1.3.3 | 0.02 |
- |
N-acetyl-L-neuraminic acid |
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C |
666750 |
| 4.1.3.3 | 0.023 |
- |
N-acetylneuraminate |
purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C |
727317 |
| 4.1.3.3 | 0.032 |
- |
N-acetylneuraminate |
mutant enzyme G211S, at pH 7.0 and 37°C |
749150 |
