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Commentary

2.1.1.56

0.000054

S-adenosyl-L-methionine

pH 7.5, 20°C

721099

2.1.1.56

0.0001947

G(5')pppR-RNA

pH 7.5, 20°C

721099

2.1.1.56

0.0018

G(5')pppR-RNA

mutant enzyme K607R, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C

706721

2.1.1.56

0.0022

G(5')pppR-RNA

mutant enzyme E763A, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C

706721

2.1.1.56

0.003

G(5')pppR-RNA

mutant enzyme F679H, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C; mutant enzyme N570D, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C

706721

2.1.1.56

0.0035

G(5')pppR-RNA

mutant enzyme F679N, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C

706721

2.1.1.56

0.004

G(5')pppR-RNA

mutant enzyme F679I, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C; mutant enzyme F679V, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C

706721

2.1.1.56

0.0057

G(5')pppR-RNA

mutant enzyme N570A, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C

706721

2.1.1.56

0.007

S-adenosyl-L-methionine

mutant enzyme N570A, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C

706721

2.1.1.56