EC Number   |
Turnover Number Minimum [1/s]  |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    1.8.4.9 | 0.00075 |
- |
3'-phosphoadenosine 5'-phosphosulfate |
- |
687585 |
| 1.8.4.9 | 0.012 |
- |
3'-phosphoadenosine-5'-phosphosulfate |
assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C |
667703 |
| 1.8.4.9 | 0.153 |
- |
5'-adenylylsulfate |
assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C |
667703 |
| 1.8.4.9 | 0.176 |
- |
adenosine 5'-phosphosulfate |
- |
687585 |
| 1.8.4.9 | 2.1 |
- |
5'-adenylylsulfate |
with glutathione as reductant |
288642 |
| 1.8.4.9 | 3.4 |
- |
5'-adenylylsulfate |
with DTT as reductant |
288642 |
| 1.8.4.9 | 8.4 |
- |
5'-adenylyl sulfate |
30°C, pH 8.5, cosubstrate: dithiothreitol |
689431 |
| 1.8.4.9 | 8.4 |
- |
glutathione |
30°C, pH 8.5 |
689431 |
| 1.8.4.9 | 10.1 |
- |
dithiothreitol |
30°C, pH 8.5 |
689431 |
| 1.8.4.9 | 11.3 |
- |
5'-adenylyl sulfate |
30°C, pH 8.5, cosubstrate: glutathione |
689431 |
