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Results 1 - 10 of 11 > >>
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Reference
0.00075
-
3'-phosphoadenosine 5'-phosphosulfate
-
0.012
-
3'-phosphoadenosine-5'-phosphosulfate
assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25C
0.153
-
5'-adenylylsulfate
assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25C
0.176
-
adenosine 5'-phosphosulfate
-
2.1
-
5'-adenylylsulfate
with glutathione as reductant
3.4
-
5'-adenylylsulfate
with DTT as reductant
8.4
-
5'-adenylyl sulfate
30C, pH 8.5, cosubstrate: dithiothreitol
8.4
-
glutathione
30C, pH 8.5
10.1
-
dithiothreitol
30C, pH 8.5
11.3
-
5'-adenylyl sulfate
30C, pH 8.5, cosubstrate: glutathione
Results 1 - 10 of 11 > >>