EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.5.3.5 | 0.1 |
- |
(S)-nicotine |
wild-type, pH 7.4, 22°C |
764029 |
1.5.3.5 | 0.26 |
- |
(S)-6-hydroxynicotine |
pH 7.0, 25°C, mutant enzyme K287M |
741943 |
1.5.3.5 | 1.14 |
- |
(S)-nicotine |
mutant R274A/Y311W/C417W, pH 7.4, 37°C |
764029 |
1.5.3.5 | 1.44 |
- |
(S)-nicotine |
mutant Y311W, pH 7.4, 22°C |
764029 |
1.5.3.5 | 2.03 |
- |
(S)-nicotine |
pH 7.0, 40°C |
741686 |
1.5.3.5 | 2.5 |
- |
(S)-6-hydroxynicotine |
mutant R274A/Y311W/C417W, pH 7.4, 22°C |
764029 |
1.5.3.5 | 2.5 |
- |
(S)-6-hydroxynornicotine |
pH 7.0, 25°C, mutant enzyme N166A |
741943 |
1.5.3.5 | 2.65 |
- |
(S)-nicotine |
mutant R274A/Y311W/C417W, pH 7.4, 22°C |
764029 |
1.5.3.5 | 4.8 |
- |
(S)-6-hydroxynicotine |
pH 7.0, 25°C, mutant enzyme N166A |
741943 |
1.5.3.5 | 7.3 |
- |
(S)-6-hydroxynicotine |
pH 7.0, 40°C |
741686 |