EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.1.87 | 0.099 |
- |
D-malate |
wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.099 |
- |
NAD+ |
wild type enzyme, with D-malate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.14 |
- |
(2R,3S)-3-(4-pentenyl)malic acid |
pH 7.8, 36°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.22 |
- |
isocitrate |
mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.34 |
- |
(2R,3S)-3-allylmalic acid |
pH 7.8, 36°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.37 |
- |
isopropylmalate |
pH 7.8, 28°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.371 |
- |
3-isopropylmalate |
pH 7.8, 25°C, recombinant wild-type enzyme |
660918 |
1.1.1.87 | 0.38 |
- |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate |
recombinant His-tagged enzyme, pH 7.8, 20°C |
722303 |
1.1.1.87 | 0.38 |
- |
NAD+ |
recombinant His-tagged enzyme, pH 7.8, 20°C |
722303 |
1.1.1.87 | 0.4 |
- |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate |
native enzyme, pH 7.8, 20°C |
722303 |