EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.4.22.69 | 0.0021 |
- |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide |
mutant enzyme Q299K |
690620 |
| 3.4.22.69 | 0.0022 |
- |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide |
mutant enzyme Q200E |
690620 |
| 3.4.22.69 | 0.003 |
- |
Abz-LQSGFRK(Dnp)NH2 |
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.0034 |
- |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide |
mutant enzyme R298A |
690620 |
| 3.4.22.69 | 0.004 |
- |
Abz-SAVLQSGK(Dnp)NH2 |
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.0048 |
- |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide |
mutant enzyme R298L |
690620 |
| 3.4.22.69 | 0.0048 |
- |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide |
mutant enzyme S139A/Q299A |
690620 |
| 3.4.22.69 | 0.0069 |
- |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide |
mutant enzyme S123a/R298A |
690620 |
| 3.4.22.69 | 0.0153 |
- |
(Ala-Arg-Leu-Gln-NH)2-rhodamine |
rate of hydrolysis measured by change in absorbance at 496 nm |
668633 |
| 3.4.22.69 | 0.017 |
- |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide |
mutant enzyme DELTA(300-306) |
690620 |
