EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.1.87 | 5 |
- |
isocitrate |
wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 5 |
- |
NAD+ |
wild type enzyme, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 5.46 |
- |
homoisocitrate |
pH 7.8, 25°C, recombinant mutant A80del |
660918 |
1.1.1.87 | 6.6 |
- |
isocitrate |
mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 6.9 |
- |
homoisocitrate |
mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 9.4 |
- |
3-isopropylmalate |
mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 10.9 |
- |
isocitrate |
pH 7.8, 25°C, recombinant mutant A80del |
660918 |
1.1.1.87 | 12 |
- |
homoisocitrate |
wild type enzyme, with NADP+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 12 |
- |
NADP+ |
wild type enzyme, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 13 |
- |
homoisocitrate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |