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Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
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5.2.1.5
30
60
the immobilized enzyme is more stable than the free enzyme at 30-60°C, more than 85% of its initial activity is retained after incubation for 3 h. The residual activity of the free and immobilized LA isomerase is 100% at 20°C. The free and immobilized enzymes are highly stable below 30°C. However, the activity of the free enzyme rapidly decreases with the increased temperature, and the immobilized enzyme retains its activity above 30°C. The immobilized enzyme has 81% activity at 50°C, whereas the free enzyme loses 51% of its activity
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