EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   5.1.3.30 | -999 |
- |
Co2+ slightly improves the thermostability of the enzyme |
748412 |
| 5.1.3.30 | -999 |
- |
the thermostability of the enzyme is enhanced by the addition of Mn2+ |
727226 |
| 5.1.3.30 | 20 |
60 |
activity of free DPE has small decreases after being maintained at 20-40°C, and has moderate decreases at 50-60°C, while the thermal stability of the immobilized DPE increases, particularly at 50°C |
748289 |
| 5.1.3.30 | 40 |
- |
2 h, 46% loss of activity |
727657 |
| 5.1.3.30 | 40 |
- |
4 h, stable |
727229 |
| 5.1.3.30 | 40 |
- |
purified recombinant His6-tagged enzyme, over 75% activity remaining after 4 h |
748499 |
| 5.1.3.30 | 40 |
- |
recombinant enzyme in overexpressing Escherichia coli cells, pH 6.5, 64.7%, activity remaining after 18 h |
748501 |
| 5.1.3.30 | 40 |
70 |
the purified recombinant enzyme retains 70% and 30% of its initial activity after 4 h of incubation at 40°C and 50°C, respectively, 55% activity remaining after 0.5 h at 60°C, complete inactivation after 0.5 h at 70°C |
748412 |
| 5.1.3.30 | 45 |
- |
in the absence of Co2+, the activity is very stable below 45 °C but decreases at temperatures above 50 °C with increasing reaction times. When incubated with 0.4 mM Co2+, the half-lives (t1/2) of the enzyme are extended |
726795 |
| 5.1.3.30 | 50 |
- |
2 h, 42% loss of activity, thermostability property is almost not affected by the addition of Co2+ |
727657 |
