EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    4.3.1.19 | 0 |
- |
10 h, 81% loss of activity in presence of 0.05 mM Ile, stable in presence of 1 mM Ile |
210829 |
| 4.3.1.19 | 27 |
- |
10 h, 27% loss of activity in presence of 0.05 mM Ile, stable in presence of 1 mM Ile |
210829 |
| 4.3.1.19 | 37 |
- |
10 min, 16% loss of activity of dehydratase I and 36% loss of activity of dehydratase II, loss of activity of dehydratase I is prevented by 1 mM Ile, but that of dehydratase II is not |
210822 |
| 4.3.1.19 | 39 |
- |
15 min, 50% loss of activity, wild-type enzyme |
747392 |
| 4.3.1.19 | 41 |
- |
15 min, 50% loss of activity, mutant enzyme A14T |
747392 |
| 4.3.1.19 | 41.2 |
- |
15 min, 50% loss of activity, mutant enzyme R449C |
747392 |
| 4.3.1.19 | 42 |
- |
15 min, 50% loss of activity, mutant enzyme T344A |
747392 |
| 4.3.1.19 | 42 |
- |
half-life: 10 min (wild-type enzyme), 210 min (mutant enzyme G323D/F510L/T344A), 14 min (mutant enzyme A14T), 19 min (mutant enzyme T344A), 15 min (mutant enzyme R449C), 57 min (mutant enzyme G323D), 44 min (mutant enzyme F510L), 54 min (mutant enzyme A14T/G323D and mutant enzyme G323D/R449C), 61 min (mutant enzyme G323D/T344A), 201 min (mutant enzyme G323D/F510L), 200 min (mutant enzyme G323D/F510L/R449C), 211 min (mutant enzyme G323D/F510L/T344A/R449C) |
747392 |
| 4.3.1.19 | 43 |
- |
T50: 43°C |
730759 |
| 4.3.1.19 | 44.8 |
- |
15 min, 50% loss of activity, mutant enzyme F510L |
747392 |
