EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   4.1.1.76 | 30 |
- |
below, 10 min, stable |
4830 |
| 4.1.1.76 | 30 |
45 |
thermal stability of the enzyme is examined in the range of 30-45°C by measuring the residual activity after a 10 min incubation at specified temperatures using phenylmalonate as the substrate. The enzyme retains 85% activity after incubation at 30°C. The activity dramatically decreases after incubation at 35°C, and it is almost negligible after incubation at 45°C |
690571 |
| 4.1.1.76 | 30 |
60 |
retains 100% activity at 30°C, activity is almost negligible at 60°C |
680955 |
| 4.1.1.76 | 40 |
- |
10 min, about 10% loss of activity |
4830 |
| 4.1.1.76 | 50 |
- |
10 min, about 50% loss of activity |
4830 |
| 4.1.1.76 | 50 |
- |
mutant enzymes Cys101Ser, Cys148Ser, Cys171Ser and Cys188Ser, half-life: 12-19 min |
4832 |
| 4.1.1.76 | 50 |
- |
wild-type enzyme, half-life: 32 min |
4832 |
